Sinulariapeptide F, a new peptide from culture broth of marine-derived fungus Simplicillium sp. SCSIO 41222 | The Journal of Antibiotics

Oct 25, 2024

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One new compound named sinulariapeptide F (1) together with one known butyrolactone (2) and seven known peptides (3–9) were isolated from the fungus Simplicillium sp. SCSIO 41222. Their structures and absolute configurations were established using HRESIMS, NMR spectroscopy (1H, 13C, HSQC, HMBC) and marfey’s method. All of these compounds were assessed their inhibitory activity of acetylcholinesterase (AChE) and pancreatic lipase (PL). Compounds 4 and 6 were selected to test for the inhibitory activity against programmed cell death-1 (PD-1)/ programmed cell death-ligand 1 (PD-L1). The results indicated that compound 4 displayed potent inhibition activity against PD-1/ PD-L1 with an IC50 value of 0.656 μM. Furthermore, the docking analysis demonstrated the interactions between 4 and proteins, suggesting PD-L1 to be a probable target for compound 4.

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This research was funded by the National Natural Science Foundation of China, grant number 42276128, and U23A20528, Guangdong Basic and Applied Basic Research Foundation, grant number 2024A1515010741. We are grateful to Dr. Z. Xiao, A. Sun, X. Zhen, X. Ma, and Y. Zhang in the analytical facility at SCSIO for recording spectroscopic data.

These authors contributed equally: Yan-Chun He, Meng-Qin Wang

Chinese Academy of Sciences (CAS) Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, CAS, Guangzhou, 510301, China

Yan-Chun He, Yong-Hong Liu & Bin Yang

University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, P. R. China

Yan-Chun He & Yong-Hong Liu

Pharmacy School of Guilin Medical University, Guilin, 541004, P. R. China

Meng-Qin Wang, Qing-Qing Tie, Xiao-Wen Huang & Yun-Qiu Li

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Correspondence to Yun-Qiu Li or Bin Yang.

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He, YC., Wang, MQ., Tie, QQ. et al. Sinulariapeptide F, a new peptide from culture broth of marine-derived fungus Simplicillium sp. SCSIO 41222. J Antibiot (2024). https://doi.org/10.1038/s41429-024-00780-w

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Received: 25 June 2024

Revised: 28 August 2024

Accepted: 02 October 2024

Published: 25 October 2024

DOI: https://doi.org/10.1038/s41429-024-00780-w

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